|First appearance: Night 2|
“ Hello... Oh! Hey, Jake, it's me, Lisa! ”
— Lisa introducing herself
Lisa Bertsch is the recorder of the 2nd Night's message. She explains to the player that Photo Negative Mickey CAN see but not very well and relies much more on hearing. She also warns the player of Oswald and explains that he is incomplete and is lacking arms and color, which makes it harder to spot Oswald. Lisa might also be Jake's friend because of the way she introduces herself to him. However, this may not be true, because when Lisa e-mails Greg about Jake, she seems to not know much about him.
- Lisa is one of four female characters in-game, the others being UO, Daisy Duck, and Impure Mouse.
- Lisa is the only female of the game who isn't an enemy.
- Lisa's full name was confirmed in TGsEE.