Night 2 is the second night of Five Nights at Treasure Island. Willy and Oswald are noticably more active on this night. During this night, Lisa calls, explaining that the suits aren't actually suits, and that they are not sure what they are. She also tells the player to keep an eye out for Oswald. She also warns the player that the suits can see, and she warns the player not to enter Pirate Caverns. Willy and Oswald are more active on this night, and Impure Mouse becomes active.

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